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epoxysilane-coated microarray slides nexterion slide e  (SCHOTT)

 
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    SCHOTT epoxysilane-coated microarray slides nexterion slide e
    Epoxysilane Coated Microarray Slides Nexterion Slide E, supplied by SCHOTT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+slides+coated+with+epoxysilane+nexterion+slide+e/epoxy+coated+slides+schott+nexterion/pm37432214-57-6-11
    Average 90 stars, based on 1 article reviews
    epoxysilane-coated microarray slides nexterion slide e - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: Fibrinogen Fucosylation as a Prognostic Marker of End-Stage Renal Disease in Patients on Peritoneal Dialysis
    Article Snippet: .. Fibrinogen samples, diluted in 50 mM PBS pH 7.4 to 100 μg/mL, were printed onto microarray slides coated with epoxysilane (NEXTERION Slide E, Schott, Germany), in triplicate into eight identical subarrays using a non-contact piezoelectric printer sciFLEXARRAYER S1 and piezo dispense capillary PDC 90 (Scienion AG, Berlin, Germany), at a temperature of 14 °C and humidity of 60%, and incubated at 4 °C for 2 h. Unoccupied epoxy groups were blocked with 3% BSA in PBS, at 4 °C for 1 h. After washing, printed proteins were incubated with biotinylated lectins ( ) at the concentration of 25 μg/mL in PBS with 0.05% Tween 20 (PBST), at 25 °C for 1 h. All lectins were purchased (Vector, Burlingame, CA, USA) except PhoSL which was a kind gift from Dr. Yuka Kobayashi (J-Oil Mills Inc., Yokohama, Japan). .. After thorough washing, bound lectins were allowed to interact with 0.5 μg/mL CF647-streptavidin conjugate (Biotium, Hayward, CA, USA) in PBST at 25 °C for 15 min.

    Article Title: Glycoanalysis of the placental membrane glycoproteins throughout placental development.
    Article Snippet: Structural changes of glycans are observed in different (patho)physiological conditions.. Human placental membrane (glyco)proteins were isolated from the first and third trimester placentas of mothers at different ages.. By using lectin microarray, we demonstrated that the placental membrane N-glycome contains several N-glycan groups: high mannose, asialylated and sialylated biantennary moieties, bisected, core fucosylated, fucosylated at other positions (bearing terminal and/or antennary Fuc), α2-6 and α2-3 sialylated structures.

    Incubation:

    Article Title: Fibrinogen Fucosylation as a Prognostic Marker of End-Stage Renal Disease in Patients on Peritoneal Dialysis
    Article Snippet: .. Fibrinogen samples, diluted in 50 mM PBS pH 7.4 to 100 μg/mL, were printed onto microarray slides coated with epoxysilane (NEXTERION Slide E, Schott, Germany), in triplicate into eight identical subarrays using a non-contact piezoelectric printer sciFLEXARRAYER S1 and piezo dispense capillary PDC 90 (Scienion AG, Berlin, Germany), at a temperature of 14 °C and humidity of 60%, and incubated at 4 °C for 2 h. Unoccupied epoxy groups were blocked with 3% BSA in PBS, at 4 °C for 1 h. After washing, printed proteins were incubated with biotinylated lectins ( ) at the concentration of 25 μg/mL in PBS with 0.05% Tween 20 (PBST), at 25 °C for 1 h. All lectins were purchased (Vector, Burlingame, CA, USA) except PhoSL which was a kind gift from Dr. Yuka Kobayashi (J-Oil Mills Inc., Yokohama, Japan). .. After thorough washing, bound lectins were allowed to interact with 0.5 μg/mL CF647-streptavidin conjugate (Biotium, Hayward, CA, USA) in PBST at 25 °C for 15 min.

    Concentration Assay:

    Article Title: Fibrinogen Fucosylation as a Prognostic Marker of End-Stage Renal Disease in Patients on Peritoneal Dialysis
    Article Snippet: .. Fibrinogen samples, diluted in 50 mM PBS pH 7.4 to 100 μg/mL, were printed onto microarray slides coated with epoxysilane (NEXTERION Slide E, Schott, Germany), in triplicate into eight identical subarrays using a non-contact piezoelectric printer sciFLEXARRAYER S1 and piezo dispense capillary PDC 90 (Scienion AG, Berlin, Germany), at a temperature of 14 °C and humidity of 60%, and incubated at 4 °C for 2 h. Unoccupied epoxy groups were blocked with 3% BSA in PBS, at 4 °C for 1 h. After washing, printed proteins were incubated with biotinylated lectins ( ) at the concentration of 25 μg/mL in PBS with 0.05% Tween 20 (PBST), at 25 °C for 1 h. All lectins were purchased (Vector, Burlingame, CA, USA) except PhoSL which was a kind gift from Dr. Yuka Kobayashi (J-Oil Mills Inc., Yokohama, Japan). .. After thorough washing, bound lectins were allowed to interact with 0.5 μg/mL CF647-streptavidin conjugate (Biotium, Hayward, CA, USA) in PBST at 25 °C for 15 min.

    Protein Concentration:

    Article Title: Glycoanalysis of the placental membrane glycoproteins throughout placental development.
    Article Snippet: Structural changes of glycans are observed in different (patho)physiological conditions.. Human placental membrane (glyco)proteins were isolated from the first and third trimester placentas of mothers at different ages.. By using lectin microarray, we demonstrated that the placental membrane N-glycome contains several N-glycan groups: high mannose, asialylated and sialylated biantennary moieties, bisected, core fucosylated, fucosylated at other positions (bearing terminal and/or antennary Fuc), α2-6 and α2-3 sialylated structures.



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    Schematic representation of intrinsic and extrinsic factors influencing <t>microarray</t> analysis of CBP–carbohydrate interactions. The color coding in the background from red to white (from top right to bottom left) indicates the extent of research work assessing these parameters.
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    microarray epoxysilane-coated slides nexterion® slide e  (SCHOTT)
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    epoxysilane coated microarray slides nexterion slide e  (SCHOTT)
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    Image Search Results


    Schematic representation of intrinsic and extrinsic factors influencing microarray analysis of CBP–carbohydrate interactions. The color coding in the background from red to white (from top right to bottom left) indicates the extent of research work assessing these parameters.

    Journal: Sensors (Basel, Switzerland)

    Article Title: A Holistic 4D Approach to Optimize Intrinsic and Extrinsic Factors Contributing to Variability in Microarray Biosensing in Glycomics

    doi: 10.3390/s23125362

    Figure Lengend Snippet: Schematic representation of intrinsic and extrinsic factors influencing microarray analysis of CBP–carbohydrate interactions. The color coding in the background from red to white (from top right to bottom left) indicates the extent of research work assessing these parameters.

    Article Snippet: The microarray slides with epoxysilane coating NEXTERION ® E were bought from SCHOTT MINIFAB (Jena, Germany).

    Techniques: Microarray

    4D approach adopted to optimize factors contributing to variability in microarray analysis of CBP–carbohydrate interactions. The chronological order of the four major steps in optimization follows the solid arrow heads from Design to Dispense, Detect and Deduce.

    Journal: Sensors (Basel, Switzerland)

    Article Title: A Holistic 4D Approach to Optimize Intrinsic and Extrinsic Factors Contributing to Variability in Microarray Biosensing in Glycomics

    doi: 10.3390/s23125362

    Figure Lengend Snippet: 4D approach adopted to optimize factors contributing to variability in microarray analysis of CBP–carbohydrate interactions. The chronological order of the four major steps in optimization follows the solid arrow heads from Design to Dispense, Detect and Deduce.

    Article Snippet: The microarray slides with epoxysilane coating NEXTERION ® E were bought from SCHOTT MINIFAB (Jena, Germany).

    Techniques: Microarray

    The effect of blocking buffer composition on signal-to-noise-ratio values of the interaction of lectin ConA with serum and fetuin printed on the microarray slide (1-drop volume, 50 μg/mL). Error bars indicate standard error ( n = 10). The microarray slides were blocked with the eight buffers described in .

    Journal: Sensors (Basel, Switzerland)

    Article Title: A Holistic 4D Approach to Optimize Intrinsic and Extrinsic Factors Contributing to Variability in Microarray Biosensing in Glycomics

    doi: 10.3390/s23125362

    Figure Lengend Snippet: The effect of blocking buffer composition on signal-to-noise-ratio values of the interaction of lectin ConA with serum and fetuin printed on the microarray slide (1-drop volume, 50 μg/mL). Error bars indicate standard error ( n = 10). The microarray slides were blocked with the eight buffers described in .

    Article Snippet: The microarray slides with epoxysilane coating NEXTERION ® E were bought from SCHOTT MINIFAB (Jena, Germany).

    Techniques: Blocking Assay, Microarray

    The effect of blocking time on the signal intensities of the interactions of lectin ConA with serum and fetuin printed on the microarray slide (1-drop volume, 50 μg/mL). Error bars indicate standard error ( n = 10).

    Journal: Sensors (Basel, Switzerland)

    Article Title: A Holistic 4D Approach to Optimize Intrinsic and Extrinsic Factors Contributing to Variability in Microarray Biosensing in Glycomics

    doi: 10.3390/s23125362

    Figure Lengend Snippet: The effect of blocking time on the signal intensities of the interactions of lectin ConA with serum and fetuin printed on the microarray slide (1-drop volume, 50 μg/mL). Error bars indicate standard error ( n = 10).

    Article Snippet: The microarray slides with epoxysilane coating NEXTERION ® E were bought from SCHOTT MINIFAB (Jena, Germany).

    Techniques: Blocking Assay, Microarray

    The effect of lectin incubation times on the signal intensities of the interaction of lectin ConA with serum (green bars) and fetuin (orange bars) printed on the microarray slide (1-drop volume, 100 μg/mL). Error bars indicate standard error ( n = 10).

    Journal: Sensors (Basel, Switzerland)

    Article Title: A Holistic 4D Approach to Optimize Intrinsic and Extrinsic Factors Contributing to Variability in Microarray Biosensing in Glycomics

    doi: 10.3390/s23125362

    Figure Lengend Snippet: The effect of lectin incubation times on the signal intensities of the interaction of lectin ConA with serum (green bars) and fetuin (orange bars) printed on the microarray slide (1-drop volume, 100 μg/mL). Error bars indicate standard error ( n = 10).

    Article Snippet: The microarray slides with epoxysilane coating NEXTERION ® E were bought from SCHOTT MINIFAB (Jena, Germany).

    Techniques: Incubation, Microarray

    The effect of dye incubation times on signal intensities of the interaction of lectin ConA with serum (green bars and fetuin (orange bars) printed on microarray slide (1-drop volume, 100 μg/mL). Error bars indicate standard error ( n = 10).

    Journal: Sensors (Basel, Switzerland)

    Article Title: A Holistic 4D Approach to Optimize Intrinsic and Extrinsic Factors Contributing to Variability in Microarray Biosensing in Glycomics

    doi: 10.3390/s23125362

    Figure Lengend Snippet: The effect of dye incubation times on signal intensities of the interaction of lectin ConA with serum (green bars and fetuin (orange bars) printed on microarray slide (1-drop volume, 100 μg/mL). Error bars indicate standard error ( n = 10).

    Article Snippet: The microarray slides with epoxysilane coating NEXTERION ® E were bought from SCHOTT MINIFAB (Jena, Germany).

    Techniques: Incubation, Microarray

    Relative intensity values of interaction of lectin ConA with serum printed on microarray slide (-drop volume, 100 μg/mL) at storage conditions (1) after 8 h at 25 °C; (2) after 24 h at −20 °C, blocked and sealed; (3) after 24 h at −20 °C, sealed without blocking; (4) after 24 h at 4 °C, without blocking or vacuum sealing. Error bars indicate standard error ( n = 10).

    Journal: Sensors (Basel, Switzerland)

    Article Title: A Holistic 4D Approach to Optimize Intrinsic and Extrinsic Factors Contributing to Variability in Microarray Biosensing in Glycomics

    doi: 10.3390/s23125362

    Figure Lengend Snippet: Relative intensity values of interaction of lectin ConA with serum printed on microarray slide (-drop volume, 100 μg/mL) at storage conditions (1) after 8 h at 25 °C; (2) after 24 h at −20 °C, blocked and sealed; (3) after 24 h at −20 °C, sealed without blocking; (4) after 24 h at 4 °C, without blocking or vacuum sealing. Error bars indicate standard error ( n = 10).

    Article Snippet: The microarray slides with epoxysilane coating NEXTERION ® E were bought from SCHOTT MINIFAB (Jena, Germany).

    Techniques: Microarray, Blocking Assay